David N. Langelaan
Are you interested in protein characterization and engineering?
We are! A large focus of my research group involves using biophysical techniques and functional assays to characterize the fundamental causes and consequences of protein assembly. Two of our main research areas are described below. If you are interested in joining our team, please contact David Langelaan.
Hydrophobin characterization and engineering
Hydrophobins are small proteins that are ubiquitously expressed by filamentous fungi. These proteins are extremely surface active, form monolayers at interfaces and also structurally rearrange to become a functional amyloid that can be deposited onto surfaces. Due to their unique properties, hydrophobins have potential uses in industry. With the recent explosion of genomic data that is available, many new hydrophobin-coding sequences have been discovered that are not well classified or characterized. The goal of our lab is to gain mechanistic understanding into hydrophobin function by characterizing new hydrophobins and determining the mechanisms of their assembly. Furthermore, we are actively engineering hydrophobins to control their properties for commercial applications.
Deciphering the role of MITF in development and melanoma
The microphthalmia family (MiT) of transcription factors are DNA-binding proteins that are responsible for recruiting transcription machinery and controlling expression of specific genes. They have important regulatory roles in a variety of cellular processes including pluripotency, homeostasis and cell differentiation. In particular, one member of the MiT family, MITF, is specifically expressed in melanocytes and is a master regulator of melanocyte development. Because of this critical role, mutation of MITF is implicated in diseases such as Waardenburg Syndrome type 2, Tietz syndrome and melanoma, where it serves as a lineage addiction oncogene. Our goal is to biophysically characterize the protein-protein interactions and post-translational modifications of MITF in both healthy and diseased states. This will allow us to gain an understanding of the mechanisms of MITF signalling and provide new routes of therapeutic intervention to treat MITF related diseases.
Gene cloning and mutagenesis, protein expression and purification, NMR spectroscopy, X-ray crystallography, isothermal titration calorimetry, fluorescence spectroscopy
|Kyle Lynch||Biochemistry (2018)|
|Connor Cranstone||Research Assistant|
|Kyle Lynch||Research Assistant|
- Langelaan, D.N., Pandey, A., Sarker, M. and Rainey, J.K., (2017) Preserved transmembrane segment topology, structure, and dynamics in disparate micellar environments J Phys Chem Lett 8:2381-2386 [PubMed] [Article]
- Grondin, J.M, Langelaan, D.N, Smith S.P., (2017) Quantifying protein-carbohydrate interactions by NMR. Methods in Molecular Biology, Wade Abbott Springer-Verlag, New York:143-156 [PubMed]
- Conroy, B.S., Weiss, E.R., Smith, S.P. and Langelaan, D.N., (2017) Backbone (1)H, (13)C, and (15)N NMR resonance assignments of the Krüppel-like factor 4 activation domain Biomol NMR Assign 11(1):95-98 [PubMed]
- Gandier, J.A., Langelaan, D.N., Won, A., O'Donnell, K., Grondin, J.L., Spencer, H.L., Wong, P., Tillier, E., Yip, C., Smith, S.P. and Master, E.R., (2017) Characterization of a Basidiomycota hydrophobin reveals the structural basis for a high-similarity Class I subdivision Sci. Rep. 10(7):45863 [PubMed]
- Ding Y., Nash J., Berezuk A., Khursigara C.M., Langelaan D.N., Smith S.P., and Jarrell K.F., (2016) Identification of the First Transcriptional Activator of an Archaellum Operon in a Euryarchaeon. Mol. Microbiol. 102:54-70 [PubMed]
- Langelaan, D.N., Liburd, J., Yang, Y., Miller, E., Chitayat, S., Crawley, S.W., Côté, G.P. and Smith, S.P. , (2016) Structure of the single-lobe myosin light chain C in complex with the light chain-binding domains of myosin-1C provides insights into divergent IQ-motif recognition J. Biol. Chem. 291:19607-17 [PubMed]
- Read J., Clancy E.K., Sarker M., de Antueno R., Langelaan D.N., Parmar H.B., Shin K., Rainey J.K., and Duncan R., (2015) Reovirus FAST Proteins Drive Pore Formation and Syncytiogenesis Using a Novel Helix-Loop-Helix Fusion-Inducing Lipid Packing Sensor. PLoS Pathog. 11(6): [PubMed]
- Denis C.M., Langelaan D.N., Kirlin A.C., Chitayat S., Munro K., Spencer H.L., LeBrun D.P., and Smith S.P., (2014) Functional redundancy between the transcriptional activation domains of E2A is mediated by binding to the KIX domain of CBP/p300. Nucleic Acids. Res. 42(11):7370-7382 [PubMed]
- Langelaan, D.N., Reddy, T., Banks, A.W., Dellaire, G., Dupré, D. and Rainey, J.K., (2013) Structural features of the apelin receptor N-terminal tail and first transmembrane segment implicated in ligand binding and receptor trafficking. Biochimica et Biophysica Acta - Biomembranes 1828:1471-1483 [PubMed] [Article]
- Langelaan, D.N.*, Ngweniform, P.*, Rainey, J.K. (*contributed equally), (2011) Biophysical characterization of G-protein coupled receptor-peptide ligand binding. Biochem Cell Biol 89:98-105 [PubMed]
- Langelaan, D.N. and Rainey, J.K., (2010) Membrane catalysis of peptide-receptor binding. Biochem Cell Biol 88:203-210 [PubMed]
- Langelaan, D.N., Wieczorek, M., Blouin, C. and Rainey, J.K., (2010) Improved helix and kink characterization in membrane proteins allows evaluation of kink sequence predictors. J. Chem. Inf. Model. 50:2213-2220 [PubMed]
- Langelaan, D.N., Bebbington, E.M., Reddy, T. and Rainey, J.K., (2009) Structural insight into G-protein coupled receptor binding by apelin Biochemistry 48:537-548 [PubMed]
- Langelaan, D.N. and Rainey, J.K., (2009) Headgroup-dependent membrane catalysis of apelin-receptor interactions is likely. J Phys Chem B 113:10465-10471 [PubMed]